Name: Saggio combinato di amplificazione della polimerasi ricombinata CRISPR/Cas12a per il rilevamento di Fusarium oxysporum f. sp. cubense Tropical Race 4 (Video) | JoVE Journal
Uploaded: 2025-11-14T13:35:00.000+00:00
Duration: 6 min 47 s
Description: Scopri un saggio combinato di amplificazione della polimerasi ricombinata e CRISPR/Cas12a per il rilevamento rapido di Fusarium oxysporum f. sp. cubense Tropical Race 4 nelle colture di banane.

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<li>Matthews, M. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+combined+recombinase+polymerase+amplification+CRISPR%2FCas12a+assay+for+detection+of+Fusarium+oxysporum+f.+sp.+cubense+tropical+race+4%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">A combined recombinase polymerase amplification CRISPR/Cas12a assay for detection of Fusarium oxysporum f. sp. cubense tropical race 4.</a> Sci Rep. 15 (1), 2436(2025).</li>
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<li>Zhang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+and+sensitive+detection+of+toxigenic+Fusarium+asiaticum+integrating+recombinase+polymerase+amplification%2C+CRISPR%2FCas12a%2C+and+lateral+flow+techniques%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Rapid and sensitive detection of toxigenic Fusarium asiaticum integrating recombinase polymerase amplification, CRISPR/Cas12a, and lateral flow techniques.</a> Int J Mol Sci. 24 (18), 14134(2023).</li>
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</ol>

<table><tbody><tr><td>Proteina EnGen Lba Cas12a (Cfp1)</td><td>New England Biolabs</td><td>M0653T</td><td></td></tr><tr><td>Diluente EnGen Lba Cas12a</td><td>New England Biolabs</td><td>B0653A</td><td></td></tr><tr><td>Tubi PCR Eppendorf</td><td>Merck</td><td>EP0030124332</td><td></td></tr><tr><td>Provette micro a sicurezza Eppendorf</td><td>Merck</td><td>EP0030121708</td><td></td></tr><tr><td>Sacchi di estrazione universali</td><td>BIOREBA</td><td>430100</td><td></td></tr><tr><td>GeneRuler 100 bp Scala del DNA</td><td>Thermo Fischer Scientific</td><td>SM0241 / SM0242</td><td></td></tr><tr><td>Centrifuga Genie Mini-6KO</td><td>OptiGene</td><td>OP-FUGE</td><td></td></tr><tr><td>Genie III</td><td>OptiGene</td><td>https://www.optigene.co.uk/instruments/genie-iii/&nbsp;</td><td></td></tr><tr><td>Portastriscie Genie</td><td>OptiGene</td><td>GBLOCK-01 / GBLOCK-02 / GBLOCK-03</td><td></td></tr><tr><td>Strisce Genie</td><td>OptiGene</td><td>OP-0008-50 / OP-0008-500</td><td></td></tr><tr><td>Kit di sintesi di RNA ad alta resa HiScribe T7</td><td>New England Biolabs</td><td>E2040S</td><td></td></tr><tr><td>NanoDrop ND-1000 Spettrofotometro UV/Vis</td><td>Thermo Fischer Scientific</td><td>https://caeonline.com/buy/spectrometers/nanodrop-nd-1000/293669253</td><td></td></tr><tr><td>NEBuffer 3</td><td>New England Biolabs</td><td>B7003S</td><td></td></tr><tr><td>NEBuffer r2.1</td><td>New England Biolabs</td><td>B6002S</td><td></td></tr><tr><td>Policilpirridone (PVP) in polvere</td><td>Sigma-Aldrich</td><td>9003-39-8</td><td></td></tr><tr><td>Kit RNA Clean e Concentrator-5</td><td>Zymo Research</td><td>R1013</td><td></td></tr><tr><td>Colorante di carico di RNA</td><td>Thermo Fischer Scientific</td><td>R0641</td><td></td></tr><tr><td>Trasilluminatore SmartBlue Blue Light</td><td>Strumenti Accuris</td><td>E4000-E</td><td></td></tr><tr><td>Pellet di idrossido di sodio (NaOH)</td><td>Merck, EMPARTA</td><td>K277</td><td></td></tr><tr><td>Tris (idrossimetil) amminatomano</td><td>Merck</td><td>77-86-1</td><td></td></tr><tr><td>Kit base liquido TwistAmp</td><td>TwistDx</td><td>TALQBAS01</td><td></td></tr></tbody></table>

saggio combinato di amplificazione della polimerasi ricombinata crispr/cas12a per il rilevamento di fusarium oxysporum f. sp. cubense tropical race 4

Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4

Saggio combinato di amplificazione della polimerasi ricombinata CRISPR/Cas12a per il rilevamento di Fusarium oxysporum f. sp. cubense Tropical Race 4

Regular and accurate surveillance stands central to the efficient management of plant diseases. It can indicate which course of action is most ...

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Rilevamento del DNA in loco di parassiti tripanosomatidi e nosema ceranae attraverso lisi alcalina accoppiata al sistema RPA/CRISPR/Cas12a

Scopri un saggio combinato di amplificazione della polimerasi ricombinata e CRISPR/Cas12a per il rilevamento rapido di Fusarium oxysporum f. sp. cubense Tropical Race 4 nelle colture di banane.

Saggio combinato di amplificazione della polimerasi ricombinata CRISPR/Cas12a per il rilevamento di Fusarium oxysporum f. sp. cubense Tropical Race 4 (Video) | JoVE Journal

Saggio combinato di amplificazione della polimerasi ricombinata CRISPR/Cas12a per il rilevamento di Fusarium oxysporum f. sp. cubense Tropical Race 4

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