Name: Bidirectionele elektrische en opto-elektronische interfaces in gezonde en ischemische ex vivo rattenharten (Video) | JoVE Journal
Uploaded: 2025-07-18T13:20:00.000+00:00
Duration: 8 min 33 s
Description: Ontdek bidirectionele elektrische en opto-elektronische interfaces in gezonde en ischemische ex vivo rattenharten met behulp van het Langendorff-model. Deze methode ondersteunt bio-elektronisch onderzoek naar hartstimulatie en infarctmodellering.

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5 minutes

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</ol>

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<table><tbody><tr><td>16 Channel Headstage</td><td>Intan Technologies</td><td>C3334</td><td></td></tr><tr><td>2,3,5-Triphenyltetrazolium chloride</td><td>Sigma Aldrich</td><td>T8877-5G</td><td>trifenyltetrazoliumboorchloride </td></tr><tr><td>2-Propanol (IPA) </td><td>Fischer Scientific</td><td>A451-4</td><td></td></tr><tr><td>635 nm Laser, 500 mW</td><td>Laserglow</td><td>NA</td><td></td></tr><tr><td>Acetone</td><td>Fischer Scientific</td><td>A18-4</td><td></td></tr><tr><td>Angstrom Evovac e-beam evaporator</td><td>Angstrom</td><td>NA</td><td></td></tr><tr><td>AZ 300MIF Developer</td><td>Microchemicals</td><td>18441123163</td><td>Ontwikkelaar</td></tr><tr><td>AZ nlof 2020 </td><td>Microchemicals</td><td>1A002020</td><td></td></tr><tr><td>AZ NMP </td><td>Microchemicals</td><td>NA</td><td>Verwijderaar voor fotoresistor</td></tr><tr><td>AZ40XT-11D </td><td>Microchemicals</td><td>1000300</td><td>Fotoresistor</td></tr><tr><td>Buffered HF</td><td>Thermo Scientific</td><td>AA44627K2</td><td></td></tr><tr><td>CaCl2.2H2O</td><td>Sigma Aldrich</td><td>(C3881)</td><td></td></tr><tr><td>CO2 laser </td><td>Universal Laser Systems</td><td>VLS 4.60</td><td></td></tr><tr><td>Conductive silver paint</td><td>PELCO</td><td>16062</td><td>Leidbaar zilververf voor hechting tussen elektrode en koperdraad</td></tr><tr><td>Koperdraad met isolatie van 0,1 mm</td><td>Amazon</td><td>https://www.amazon.com/Enameled-Temperature-Resistance-Transformers-Inductors/dp/ B09TNM51S5/ref=asc_df_B09 TNM51S5?mcid=155de761ea4 c389ea86d45ba61a83e8d&amp;tag =hyprod-20&amp;linkCode=df0&amp;hvadid =693675076770&amp;hvpos=&amp;hvnetw =g&amp;hvrand=664358781094905 4431&amp;hvpone=&amp;hvptwo=&amp;hvqmt =&amp;hvdev=c&amp;hvdvcmdl=&amp;hvlocint =&amp;hvlocphy=9021716&amp;hvtargid= pla-1878323158943&amp;psc=1</td><td></td></tr><tr><td>Digidata 1550 digitizer</td><td>Molecular Devices</td><td>NA</td><td></td></tr><tr><td>Glucose</td><td>Sigma Aldrich</td><td>G7021</td><td></td></tr><tr><td>HBSS, zonder calcium, zonder magnesium, zonder fenolrood</td><td>Thermo Scientific</td><td>14175095</td><td></td></tr><tr><td>Heidelberg MLA150 direct writer</td><td>Heidelberg</td><td>NA</td><td></td></tr><tr><td>Heparine (1000 IE/kg)</td><td>Sagent Pharmaceuticals</td><td>1009655</td><td></td></tr><tr><td>HEPES</td><td>Sigma Aldrich</td><td>(H4034)</td><td></td></tr><tr><td>Hexaan </td><td>Sigma Aldrich</td><td>52750-10ML</td><td>Oplosmiddel</td></tr><tr><td>HF</td><td>Fisher Scientific</td><td>A513-500</td><td>Tracemetal grade</td></tr><tr><td>HMDS Vapor Prime Oven</td><td>Yield Engineering Systems</td><td>NA</td><td></td></tr><tr><td>HNO3</td><td>Fisher Scientific</td><td>A509P500</td><td>Tracemetal grade</td></tr><tr><td>Hetelamineerapparaat</td><td>Office Depot</td><td>L410-A</td><td>Multilayer encapsulatie </td></tr><tr><td>Intan RHD-systemen</td><td>Intan Technologies</td><td>C3100</td><td></td></tr><tr><td>iWorx ECG</td><td>iWorx</td><td>IA-400D</td><td></td></tr><tr><td>iWorx LVD probe</td><td>iWorx</td><td>BP-100</td><td></td></tr><tr><td>iWorx voorversterker</td><td>iWorx</td><td>C-ISO-256</td><td></td></tr><tr><td>KCl</td><td>Sigma Aldrich</td><td>(P4504)</td><td></td></tr><tr><td>Latexballon (maat 4)</td><td>Radnoti</td><td>170404</td><td></td></tr><tr><td>MgCl2.7H2O</td><td>Sigma Aldrich</td><td>(M9272)</td><td></td></tr><tr><td>NaCl</td><td>Sigma Aldrich</td><td>(S9625)</td><td></td></tr><tr><td>Nafion 117 met oplossing</td><td>Sigma Aldrich</td><td>31175-20-9</td><td>Fixeermiddel voor nanoporeuze koolstofpatronen</td></tr><tr><td>NaH2PO4</td><td>Sigma Aldrich</td><td>(S0751)</td><td></td></tr><tr><td>NaOH</td><td>Sigma Aldrich</td><td>221465</td><td></td></tr><tr><td>PDMS</td><td>Fisher Scientific</td><td>NC9285739</td><td></td></tr><tr><td>Plasma-Therm plasmafluoride-etser</td><td>Plasma-Therm</td><td>NA</td><td></td></tr><tr><td>PMMA (495 A6) </td><td>Microchemicals</td><td>M130006 </td><td></td></tr><tr><td>poly (pyromellitic dianhydride-co-4,4'-oxydianiline)</td><td>Sigma Aldrich</td><td>575801</td><td>PI-precursor</td></tr><tr><td>Poly(ethyleen-co-vinylacetaat)</td><td>Sigma Aldrich</td><td>437247-250G</td><td>Polymeer voor infiltratie</td></tr><tr><td>Poly(vinylalcohol)</td><td>Sigma Aldrich</td><td>341584-25G</td><td></td></tr><tr><td>Polyethyleentereftalaat (PET)</td><td>Amazon</td><td>KS-6304-21-11</td><td>Type D Doorzichtige PET-folie van 0,0005 inch dik x 27 inch breed x 10 ft lengte 1 stuk</td></tr><tr><td>Potentiostat</td><td>BioLogic</td><td>SP-200</td><td>Elektrische stimulatie</td></tr><tr><td>Weerstandsoven</td><td>Despatch</td><td>LCC1-15-5</td><td></td></tr><tr><td>Scanning electron microscopy</td><td>Carl Zeiss</td><td>Merlin</td><td>Instrument voor morfologische karakterisering</td></tr><tr><td>Silicion wafer </td><td>NOVA Electronic Materials</td><td>HS39626</td><td></td></tr><tr><td>Silicone lijm</td><td>WPI</td><td>KWIK-SIL-S</td><td>Encapsulatie</td></tr><tr><td>Natriumboraat</td><td>Sigma Aldrich</td><td>SX0355</td><td></td></tr><tr><td>SOI wafer</td><td>Ultrasil</td><td>3-10541</td><td>Type-Orient:P/B(100) DT:7+/-1um DR:&#60;.005 Ohmcm HT:300+/-10um HR:1-30 Ohmcm Box:1um+/-5% UD-13998/UH-13962</td></tr><tr><td>SU-8 2002</td><td>Microchemicals</td><td>SU-8 2000 Series</td><td></td></tr><tr><td>SU-8 ontwikkelaar</td><td>Microchemicals</td><td>SU-8 2000 Series</td><td></td></tr><tr><td>Nassgewebekleber</td><td>3M</td><td>1469SB</td><td></td></tr><tr><td>Whatman kwalitatief filterpapier, graad 1</td><td>Sigma Aldrich</td><td>WHA1001150</td><td>Substraat voor koolstofpatronen</td></tr></tbody></table>
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bidirectionele elektrische en opto-elektronische interfaces in gezonde en ischemische ex vivo rattenharten

Bidirectional Electrical and Optoelectronic Interfaces in Healthy and Ischemic Ex Vivo Rat Hearts

Bidirectionele elektrische en opto-elektronische interfaces in gezonde en ischemische ex vivo rattenharten

Electrophysiological studies are pivotal in exploring interactions between materials and cardiac biointerfaces. Recent advancements have introduced ...

bidirectional-electrical-optoelectronic-interfaces-healthy-ischemic

Research

JoVE Journal

Bioengineering

Dit artikel beschrijft een hoge temporele en ruimtelijke resolutie techniek om optisch image actie potentiaal beweging op het oppervlak van de Langendorff-geperfuseerde rattenharten met behulp van een potentiometrische kleurstof (di-8-ANEPPS).

Optisch in kaart brengen van Langendorff-geperfuseerde Rat Hearts

Dit artikel beschrijft de basisprocedures voor het uitvoeren van optische in kaart brengen van experimenten in de Langendorff-geperfuseerde konijn hart met behulp van het panoramische imaging systeem, en de dubbele (spanning en calcium) beeldvormende modaliteit.

Multiparametrische Optische kaart brengen van de Langendorff-geperfuseerde Rabbit Heart

Het doel van deze studie was het vaststellen van een methode voor het onderzoeken van de cardiale dynamiek met behulp van een translationeel diermodel. De beschreven experimentele aanpak omvat Dual-emission optocardiografie in combinatie met een elektrofysiologisch onderzoek om elektrische activiteit te beoordelen in een geïsoleerd, intact varkens hart model.

Optocardiografie en elektrofysiologie studies van ex vivo Langendorff-perfused Hearts

Dit protocol beschrijft de procedure voor het doorsnijden en kweken van menselijke cardiale plakjes voor preklinische geneesmiddelentesten en beschrijft het gebruik van optische mapping voor het opnemen van transmembranespanning en intracellulaire calciumsignalen gelijktijdig van deze plakjes.

Preklinische Cardiale Elektrofysiologie Beoordeling door Dual Voltage en Calcium Optical Mapping van human organotypic cardiale plakjes

Dit artikel presenteert een efficiënte methode om myocardiale ischemie en de daaropvolgende chronische reperfusie bij ratten uit te voeren met behulp van een minimaal invasieve aanpak. Bovendien wordt de linker ventriculaire hemodynamische functie van ratten beoordeeld door echocardiografie en geïsoleerde werkhartmethoden.

Semi-minimale invasieve methode om myocardiaal infarct induceren bij ratten en de beoordeling van de cardiale functie door een geïsoleerd werkend hartsysteem

Dit werk rapporteert een methode voor het regelen van het hartritme van intacte muizen van transgene channelrhodopsine-2 (ChR2) muizen met behulp van lokale fotostimulatie met een micro-LED-array en gelijktijdige optische mapping van epicardiale membraanpotentiaal.

Geavanceerd hartritmebeheer door toepassing van optogenetische multi-site fotostimulatie in muizenharten

Dit protocol introduceert dual-dye optische mapping van muizenharten verkregen van wildtype en knock-in dieren die zijn aangetast door catecholaminerge polymorfe ventriculaire tachycardie, inclusief elektrofysiologische metingen van transmembraanspanning en intracellulaire Ca2+ transiënten met hoge temporele en ruimtelijke resolutie.

Dual-Dye optische mapping van harten van RyR2R2474S Knock-in muizen van catecholaminerge polymorfe ventriculaire tachycardie

Dit protocol illustreert een methode voor optogenetische stimulatie van intrinsieke hartneuronen in transgene muizenharten. De beschreven benadering wordt gebruikt voor het onderzoeken van de kinetiek van plotselinge activering van hartneuronen in ex vivo geperfuseerde harten en de interacties tussen cholinerge en catecholaminerge activiteit.

Optogenetische activering van intrinsieke cardiale autonome neuronen in uitgesneden geperfuseerde muizenharten

Een 3D cultuur voor hematopoiesis beschreven met menselijke navelstrengbloed en leukemische beenmergcellen. De methode is gebaseerd op het gebruik van een poreuze kunststof polyurethaan scaffold bekleed met extracellulaire matrix. Deze steiger is aanpasbaar aan een groot aantal cellen te passen.

Ex vivo Mimicry van normale en abnormale Human Haematopoiese

Milieuvriendelijke gecontroleerde Microtensile Testen van mechanisch-adaptieve nanocomposieten voor<em&gt; Ex vivo</em&gt; Karakterisering

Ontdek bidirectionele elektrische en opto-elektronische interfaces in gezonde en ischemische ex vivo rattenharten met behulp van het Langendorff-model. Deze methode ondersteunt bio-elektronisch onderzoek naar hartstimulatie en infarctmodellering.

Bidirectionele elektrische en opto-elektronische interfaces in gezonde en ischemische ex vivo rattenharten

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