Name: Infant Immuniteit in kaart brengen met minimale input: Integratieve single-cell en multiomic profiling (Video) | JoVE Journal
Uploaded: 2026-04-03T12:35:00.000+00:00
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Description: Leer hoe je hoog-dimensionale immuunprofilering uitvoert bij pasgeborenen met minimale bloedvolumes via single-cell en multiomic technieken. Deze methode ondersteunt longitudinaal onderzoek naar vroege immuunontwikkeling.

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<li>Olin, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Stereotypic+immune+system+development+in+newborn+children%5BTitle%5D)+AND+%22Cell%22%5BJournal%5D)">Stereotypic immune system development in newborn children.</a> Cell. 174 (5), 1277-1292.e14 (2018).</li>
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</ol>

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infant immuniteit in kaart brengen met minimale input: integratieve single-cell en multiomic profiling

Mapping Infant Immunity with Minimal Input: Integrative Single-Cell and Multiomic Profiling

Infant Immuniteit in kaart brengen met minimale input: Integratieve single-cell en multiomic profiling

At birth, the neonatal immune system is abruptly confronted with a radically different environment, rich in microbial and environmental stimuli. ...

mapping-infant-immunity-with-minimal-input-integrative-single-cell

Research

JoVE Journal

Immunology and Infection

Dit protocol beschrijft hoe de expressie van een groot scala van genen op het klonale niveau te beoordelen. Single-cell RT-qPCR levert zeer betrouwbare resultaten met een sterke gevoeligheid voor honderden monsters en genen.

Eencellige genexpressie met behulp van Multiplex RT-qPCR heterogeniteit van Rare lymfoïde populaties karakteriseren

Hier beschrijven we een eencellige proteomic aanpak om te evalueren van immuun fenotypische en functionele (intracellulaire cytokine inductie) veranderingen in het perifere bloedmonsters geanalyseerd via massale cytometry.

Eencellige analyse van Immunophenotype en Cytokine productie in perifere bloed via massale Cytometry

Bulk gen expressie metingen wolk individuele cel verschillen in heterogene cel populaties. Hier beschrijven we een protocol voor hoe eencellige gen expressie analyse en index Sorteren door bloeien geactiveerd Cell Sorting (FACS) kan worden gecombineerd om te bakenen heterogeniteit en immunophenotypically karakteriseren moleculair afzonderlijke cel populaties.

Een combinatorische eencellige aanpak te karakteriseren de moleculaire en Immunophenotypic heterogeniteit van menselijke stamcellen en voorlopercellen populaties

Kwantitatieve multiplex Immunoprecipitation (QMI) gebruikt Flowcytometrie voor gevoelige detectie van verschillen in de overvloed van gerichte eiwit-eiwit interacties tussen twee monsters. QMI kan worden uitgevoerd met een kleine hoeveelheid biomateriaal, vereist geen genetisch gemanipuleerde Tags en kan worden aangepast voor elk eerder gedefinieerd proteïne-interactie netwerk.

Kwantificering van eiwit interactie netwerk dynamiek met Multiplexed co-immunoprecipitatie

Het gebruik van een RNA-gebaseerde benadering om kwantitatieve immuunprofielen van vaste tumorweefsels te bepalen en klinische cohorten te benutten voor immuun-oncologische biomarkerontdekking wordt beschreven door middel van een moleculaire en informaticaprotocollen.

Voorspellende immuunmodellering van vaste tumoren

Hier beschrijven we een eenvoudige en toegankelijke strategie voor het visualiseren, kwantificeren en in kaart brengen van immuuncellen in formaline-vaste paraffine-ingebedde tumorweefselsecties. Deze methodologie combineert bestaande beeldvormings- en digitale analysetechnieken met als doel de multiplexingcapaciteit en multiparameteranalyse van beeldvormingstesten uit te breiden.

Visualisatie, kwantificering en mapping van immuuncelpopulaties in de tumormicro-omgeving

In het tijdperk van immunotherapie en eencellige genomische profilering vereist kankerbiologie nieuwe in vitro en computationele hulpmiddelen voor het onderzoeken van de tumor-immuuninterface in een juiste spatiotemporale context. We beschrijven protocollen om tumor-immuun microfluïdische co-culturen te exploiteren in 2D- en 3D-instellingen, compatibel met dynamische, multiparametrische monitoring van cellulaire functies.

Microfluïdische co-cultuurmodellen voor het ontleden van de immuunrespons in in vitro tumormicro-omgevingen

Dit protocol combineert flowcytometrie en eencellige RNA-sequencing om microgliale celtoestanden in het cerebellum van vroege postnatale muizenhersenen te isoleren en te karakteriseren. Het maakt gebruik van enzymatische dissociatie, Percoll-centrifugatie en immunokleuring om de heterogeniteit van microglia's te onthullen en het begrip van hun rol in de ontwikkeling en ziekte van cerebellaire patiënten te verbeteren.

Flowcytometrie en eencellige analyse voor het karakteriseren van microglia-activering bij vroege postnatale hersenontwikkeling bij muizen

Een cel dood-gebaseerde test voor het PTI in Nicotiana benthamiana planten is beschreven.

Test voor Pathogeen-geassocieerde Moleculaire Pattern (PAMP)-Triggered Immuniteit (PTI) in Planten

Selectie van Plasmodium falciparum Parasieten voor Cytoadhesion Human Brain endotheelcellen

Leer hoe je hoog-dimensionale immuunprofilering uitvoert bij pasgeborenen met minimale bloedvolumes via single-cell en multiomic technieken. Deze methode ondersteunt longitudinaal onderzoek naar vroege immuunontwikkeling.

Infant Immuniteit in kaart brengen met minimale input: Integratieve single-cell en multiomic profiling

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