Name: Brillouin Spektroskopisi Kullanılarak Sferoidlerin Mekanik Haritalanması (Video) | JoVE Journal
Uploaded: 2025-12-12T13:10:00.000+00:00
Duration: 8 min 27 s
Description: Keşfedin, tümör sferoidlerinin mekanik özelliklerini 3D matriste Brillouin mikro-spektroskopisi ile nasıl karakterize edeceğinizi. Bu invaziv olmayan yöntem, mekanobiyoloji ve hastalık ilerlemesi araştırmalarında değerli bilgiler sunar.

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</ol>

<table><tbody><tr><td>4-kol PEG-Maleimid (20 kDa)</td><td>Yaratıcı PEGWorks</td><td>PSB-455</td><td>https://creativepegworks.com/product/4-arm-peg-mal-mw-20k</td></tr><tr><td>AggreWell 400 plakaları</td><td>STEMCELL Teknolojileri</td><td>34411</td><td>http://stemcell.com/products/aggrewell400.html</td></tr><tr><td>Hava aralığında etalon</td><td>LightMachinery</td><td>HF-16074-660</td><td>https://lightmachinery.com/spectrometers/brillouin-spectroscopy-excitation-laser-optimization/</td></tr><tr><td>Anti-yapışma yıkama çözeltisi</td><td>STEMCELL Teknolojileri</td><td>7010</td><td>https://www.stemcell.com/products/aggrewell-rinsing-solution.html</td></tr><tr><td>Bold Line Top Stage Kuluçka Makinesi</td><td>Okolab</td><td>H301-NIKON-NZ100/200/500-N</td><td>https://www.oko-lab.com/live-cell-imaging/stage-top-digital-gas/chamber/nikon/h301-nikon-nz100-200-500-n</td></tr><tr><td>Cobolt Flamenco-100 660 nm lazer</td><td>Huebner</td><td>0660-05-01-0100-700</td><td>https://hubner-photonics.com/products/lasers/single-frequency-lasers/05-01-series/</td></tr><tr><td>Konfokal sirkülatör</td><td>LightMachinery</td><td>HF-13311</td><td>Yok</td></tr><tr><td>D-LEDi Floresans aydınlatma sistemi&nbsp;</td><td>Nikon</td><td>MBF83000</td><td>https://www.microscope.healthcare.nikon.com/en_EU/products/light-sources/d-ledi</td></tr><tr><td>EPI-FL modülü</td><td>Nikon</td><td>Ti2-LA-FL-3</td><td>https://www.microscope.healthcare.nikon.com/en_EU/products/accessories/intermediate-modules</td></tr><tr><td>Fibronectin (insan)</td><td>YoProteins</td><td>663</td><td>https://www.yoproteins.com/fibronectin-human-5-mg-663.html</td></tr><tr><td>Gibco Dulbecco' s Modifiye Kartal Ortamı (DMEM)</td><td>ThermoFisher</td><td>11965092</td><td>https://www.thermofisher.com/order/catalog/product/11965092</td></tr><tr><td>HyperFine Brillouin spektrometresi</td><td>LightMachinery</td><td>HF-8999-PK-660</td><td>https://lightmachinery.com/spectrometers/brillouin-hyperfine-spectrometer/</td></tr><tr><td>LED-DA/FI/TR/Cy5-B Dörtlü bantlı filtre küpü</td><td>Nikon</td><td>MXR00753</td><td>https://www.microscope.healthcare.nikon.com/en_EU/products/accessories/fluorescent-filter-cubes</td></tr><tr><td>Mezenkimal kök hücreler (MSC)</td><td>PromoCell</td><td>C-12974</td><td>https://promocell.com/us_en/human-mesenchymal-stem-cells-hmsc.html</td></tr><tr><td>Motorlu çeviri aşaması&nbsp;</td><td>Önceki Bilimsel Çalışmalar</td><td>H117E2NN</td><td>https://www.prior.com/imaging-components/motorized-stages?filter=MicroscopeBrand-33</td></tr><tr><td>NucBlue&trade; Live ReadyProbes&trade; reaktif (Hoechst 33342)</td><td>ThermoFisher</td><td>R37605</td><td>https://www.thermofisher.com/order/catalog/product/R37605</td></tr><tr><td>Objective, S Plan Fluor 20X NA 0.45, hava</td><td>Nikon</td><td>MRH08230</td><td>https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1708</td></tr><tr><td>ORCA-Fusion CMOS kamera</td><td>Hamamatsu</td><td>C14440-20UP</td><td>https://www.hamamatsu.com/eu/en/product/cameras/cmos-cameras/C14440-20UP.html</td></tr><tr><td>PEG-ditiol, 2 kDa</td><td>Yaratıcı PEGWorks</td><td>PLS-613</td><td>https://creativepegworks.com/product/hs-peg-sh-mw-2k-10g</td></tr><tr><td>sCMOS kamera&nbsp;</td><td>Teledyne Vision Çözümleri</td><td>01-IRIS-15-USB-M-16-C</td><td>https://www.teledynevisionsolutions.com/en-gb/products/iris/?model=01-IRIS-15-USB-M-16-C&vertical=tvs-photometrics&segment=tvs&nbsp;</td></tr><tr><td>SPY555-aktin&nbsp;</td><td>Spirokrom</td><td>CY-SC202</td><td>https://spirochrome.com/product/spy555-actin/</td></tr><tr><td>TemperatureApp</td><td></td><td></td><td>Sıcaklık kontrol yazılımı</td></tr><tr><td>VPM peptid, GCRDVPMSMRGGDRCG</td><td>GenScript</td><td>Yok</td><td>Yok</td></tr><tr><td>Eclipse Ti-2A mikroskobu</td><td>Nikon</td><td>Eclipse Ti-2A</td><td>https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti2-series&nbsp;</td></tr></tbody></table>

brillouin spektroskopisi kullanılarak sferoidlerin mekanik haritalanması

Mechanical Mapping of Spheroids Using Brillouin Spectroscopy

Brillouin Spektroskopisi Kullanılarak Sferoidlerin Mekanik Haritalanması

Brillouin spectroscopy, an emerging technique gaining significant interest in biomedical science, allows researchers to gather information related to ...

mechanical-mapping-of-spheroids-using-brillouin-spectroscopy

Research

JoVE Journal

Bioengineering

Source: Huang, Y. et. al., Optik Koherens Tomografi Kullanarak Üç Boyutlu Tümör Sferoidlerinin Boylamsal Morfolojik ve Fizyolojik İzlemesi. J. Vis. Exp. (2019)Bu video, yüksek çözünürlüklü kesitsel görüntüler yakalayarak çok kuyulu bir plakadaki tümör sferoidlerini izlemek için optik koherens tomografinin (OCT) kullanımını göstermektedir. OCT, hücresel yapılardan gelen ışık yansımalarını analiz ederek, boyut ve şekil gibi morfolojik özellikleri değerlendirmek için 3D modeller oluştururken, aynı...

Optik Koherens Tomografi ile Tümör Sferoidlerinin Morfolojik ve Fizyolojik İzlenmesi 

Parçacık izleme microrheology olmayan yıkıcı ölçmek ve 3 boyutlu uzamsal tümör modellerinde hücre dışı matris mekanik özelliklerinde değişiklikler eşlemek için kullanılabilir.

Partikül izleme Microrheology kullanarak 3D Tümör Modelleri Ekstrasellüler Matriks Rijitliğinin Boyuna Ölçümü

Burada hızlı bir Brillouin spektrometresi inşa etmek için bir protokol mevcut. Neredeyse görüntülü faz dizi Basamaklı (VIPA) etalonları 1000'den fazla kat daha hızlı geleneksel tarama Fabry-Perot spektrometresi daha ölçüm hızına erişmek. Bu gelişme in vivo düşük güç seviyelerinde doku ve biyomalzemelerin Brillouin analizi için araçlar sağlar.

Brillouin Saçılma Analiz Yüksek Hızlı Alt GHz Spektrometre

Tam elastik özelliklerini çıkarmak için hücre dışı matrisin elastin ve tripsin saflaştırılmış tip I kollajen liflerine Brillouin ışık saçılma spektroskopisinin uygulanması için bir protokol sunuyoruz.

Brillouin Spektroskopisi için Ekstraselüler Matriks Proteini Elyaf Hazırlama

Biz bir hızlı continuous-wave-teşvik-Brillouin-saçılma (CW-SBS) Spektrometre inşaatı tarif. Spektrometre tek frekanslı diyot lazerler istihdam ve ilâ 100-fold mevcut CW-SBS Spektrometreler olanların daha hızlı bir atomik Buhar çentik-iletim spectra yüksek spektral çözünürlüklü bulanık/sigara-bulanık örneklerinin elde etmek için filtre hızlandırır. Bu geliştirme yüksek hızlı Brillouin malzeme çözümleme sağlar.<br...

Yüksek hızda sürekli uyarılan Brillouin saçılma Spektrometre için malzeme analizi

Optik koherens tomografi (OCT), üç boyutlu görüntüleme teknolojisi, izlemek ve çok hücreli tümör pulcuklarının büyüme kinetik karakterize etmek için kullanıldı. Tümör pulcuklarının yaklaşım ve içsel optik zayıflama Karşıtlık'dayalı pulcuklarının etiket içermeyen ölü dokuyu algılama sayma Voksel kullanarak hassas hacimsel miktar gösterdi.

Boyuna morfolojik ve fizyolojik izleme optik koherens tomografi kullanarak üç boyutlu tümör pulcuklarının

Fotonik bant yapısı, sınırlı elektromanyetik modların fotonik kristal içinde nasıl yayDığını anlamanızı sağlar. Manyetik elementleri içeren fotonik kristallerde, bu tür sınırlı ve rezonanslı optik modlara gelişmiş ve modifiye edilmiş manyeto-optik aktivite eşlik eder. Fourier uzay mikroskobu ile manyeto-optik bant yapısını çıkarmak için bir ölçüm prosedürü tanımladık.

Fotonik Nanoyapıların Spektral ve Açı Çözümlü Manyeto-Optik Karakterizasyonu

Protokol, standart bir floresan mikroskobunda oksijenasyonlarının ve hücre ölümlerinin çok parametrik analizini kullanarak biyobaskı için yüksek verimli sferoid üretimini tanımlar. Bu yaklaşım, sferoidlerin yaşayabilirliğini kontrol etmek ve 3D doku, tümör mikroçevresi ve başarılı (mikro) doku biyofabrikasyonunun modellenmesinde önemli olan standardizasyonu gerçekleştirmek için uygulanabilir.

Çok Hücreli Sferoidlerin Uygun Fiyatlı Oksijen Mikroskobu Destekli Biyofabrikasyonu

Bu protokol, civciv embriyo nörülasyonu sırasında nöral plak dokusunun mekanik özelliklerini uzunlamasına izlemek için geliştirilmiştir. Bir Brillouin mikroskobu ve bir sahne inkübasyon sisteminin entegrasyonuna dayanır ve ex ovo kültürlenmiş civciv embriyolarında nöral plaka dokusunun canlı mekanik görüntülemesini sağlar.

Civciv Embriyosunun Nöral Tüp Kapanması Sırasında Dokunun Mekanik Gelişiminin İzlenmesi

Raman spektroskopisi temassız için uygun bir tekniktir, canlı hücreler, doku mühendisliği yapıları ve doğal dokuların etiket ücretsiz analizidir. Kaynak özgü spektral parmak izi oluşturulur ve çok değişkenli analiz kullanılarak analiz edilebilir.

Raman Spektroskopisi kullanarak Hücreler ve Ekstrasellüler Matriks Temassız, Etiket ücretsiz izleme

Keşfedin, tümör sferoidlerinin mekanik özelliklerini 3D matriste Brillouin mikro-spektroskopisi ile nasıl karakterize edeceğinizi. Bu invaziv olmayan yöntem, mekanobiyoloji ve hastalık ilerlemesi araştırmalarında değerli bilgiler sunar.

Brillouin Spektroskopisi Kullanılarak Sferoidlerin Mekanik Haritalanması

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