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Collection Overview
This collection provides a growing resource of visual protocols using Danio rerio (zebrafish) across developmental biology, genetics, cardiovascular research, neuroscience, immunology, infection biology, cancer research, toxicology, and regenerative medicine. Current protocols cover areas including zebrafish husbandry, genome editing, microinjection, disease modeling, imaging, behavioural analysis, physiological measurements, cell isolation, and drug and environmental testing. Open to new submissions, this collection aims to serve as a practical protocol database for the zebrafish research community, bringing together reproducible methods that support both established and emerging applications of this versatile model organism.
Articles
Cytotoxicity Assays with Zebrafish Cell Lines
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Cited by 7
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2023
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Retro-orbital Injection in Adult Zebrafish
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Cited by 93
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2009
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Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish
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2025
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Xuepu Jin*1, Binghuang Zhang*1, Yu Sun*1, Yahan Duan1, Junchen Lu1, Jiannan Liu2, Jiahuai Han1,3,4, Yingying Zhang1
1State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, 2Technology Center, Shanxi Xinghuacun Fenjiu Distillery Co., Ltd., 3Research Unit of Cellular Stress of CAMS, Cancer Research Center of Xiamen University, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, 4Laboratory Animal Center, Xiamen University
A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish
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Cited by 22
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2018
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Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish
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Cited by 6
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2021
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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
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Cited by 4
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2023
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Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
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Cited by 2
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2016
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Rapid Isolation of Stage I Oocytes in Zebrafish Devoid of Granulosa Cells
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Cited by 1
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2024
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Isolation and Characterization of Single Cells from Zebrafish Embryos
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Cited by 20
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2016
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Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode
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Cited by 13
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2019
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Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos
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Cited by 57
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2015
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Audrey Bernut1,2, Christian Dupont1,2, Alain Sahuquet1, Jean-Louis Herrmann3, Georges Lutfalla1, Laurent Kremer1,2
1Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS, UMR 535, Université Montpellier, 2Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé, CNRS, FRE 3689, Université Montpellier, 3Unité de Formation et de Recherche des Sciences de la Santé, EA3647-EPIM, Université Versailles St Quentin
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
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Cited by 42
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2011
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Development of a Larval Zebrafish Infection Model for Clostridioides difficile
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Cited by 5
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2020
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1Division of Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, 2Department of Molecular Biotechnology, Turkish-German University, 3Institut für Mikrobiologie, Technische Universität Braunschweig, 4Braunschweig Integrated Centre of Systems Biology, 5Helmholtz Centre for Infection Research
Visualizing the Gatekeeper: Evan's Blue Dye-Based Assessment of Blood-Brain Barrier Permeability in Adult Zebrafish
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Cited by 2
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2025
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1Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia (IIUM), 2Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 3Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 4Clinical Pharmacology Department, Menoufia Medical School, Menoufia University
Two-Photon Calcium Imaging of Forebrain Activity in Behaving Adult Zebrafish
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Cited by 1
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2023
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Visualization of the Superior Ocular Sulcus during Danio rerio Embryogenesis
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2019
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Kevin H. Yoon1, Sonya A. Widen1,2, Melissa M. Wilson1, Jennifer C. Hocking2,3,4,5, Andrew J. Waskiewicz1,2,6
1Department of Biological Sciences, University of Alberta, 2Women & Children's Health Research Institute, University of Alberta, 3Division of Anatomy, Department of Surgery, University of Alberta, 4Department of Cell Biology, University of Alberta, 5Department of Medical Genetics, University of Alberta, 6Neuroscience and Mental Health Research Institute, University of Alberta
An Intraperitoneal Injection Technique in Adult Zebrafish that Minimizes Body Damage and Associated Mortality
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Cited by 4
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2024
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1Department of Biochemistry and Molecular Biology, Mayo Clinic, 2Department of Cardiovascular Medicine, Mayo Clinic, 3Cardiovascular Surgery Department, Guilin Hospital of 2nd Xiangya Hospital, Central South University, 4Cardiovascular Surgery Department, 2nd Xiangya Hospital, Central South University
Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction
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Cited by 320
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2012
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Avdesh Avdesh*1,2, Mengqi Chen*1,3, Mathew T. Martin-Iverson1,2,4, Alinda Mondal1,3, Daniel Ong1, Stephanie Rainey-Smith1,3, Kevin Taddei1,3, Michael Lardelli5, David M. Groth6, Giuseppe Verdile1,3, Ralph N. Martins1,2,3,7
1Centre of Excellence for Alzheimer's Disease Research and Care, School of Medical sciences, Edith Cowan University, 2Centre for Clinical Research in Neuropsychiatry, Graylands Hospital, University of Western Australia, 3McCusker Alzheimer's Research foundation, 4School of Medicine and Pharmacology, University of Western Australia , 5Department of Molecular and Biomedical Sciences, University of Adelaide, 6School of Biomedical Sciences, Curtin University of Technology, 7School of Psychiatry and Clinical Neurosciences, University of Western Australia
In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
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Cited by 24
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2012
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Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
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Cited by 11
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2016
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Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish
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Cited by 5
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2017
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X-Band Electron Paramagnetic Resonance For Monitoring Of Melanin-Associated Radical Changes In Zebrafish Melanoma Xenografts In Vivo
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2026
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Katerina Makarova1, Katarzyna Zawada1, Mikołaj Baranowski2,3, Malgorzata Korzeniowska née Wiweger1,4
1Department of Organic and Physical Chemistry, Faculty of Pharmacy, Medical University of Warsaw, 2Novilet, 3Department of Functional Materials Physics, Institute of Physics, Faculty of Physics and Astronomy, Adam Mickiewicz University, 4Laboratory of Protein Engineering, Mossakowski Medical Research Institute, Polish Academy of Sciences